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In this study, phytoliths were isolated from two different species of modern crops (rice and millet) using a modified version of the recently published protocol The extracted rice straw and millet phytoliths were examined using a scanning electron microscope (SEM) to ensure a lack of visible cellulose adhering to the outside of the phytoliths and to ensure the presence of intact samples with diameters of at least 20 μm (Supplementary Figure S1).

As shown in Supplementary Figure S1, when the temperature reaches 900°C, most phytoliths lose their distinct morphological characteristics, and when the samples are heated to 1100°C, the phytoliths disappear.